The JPBrosseauLab

Our laboratory has an interest and expertise in elucidating gene expression programs involved in tumorigenesis, especially those involving cells in the tumor microenvironment. To do this, we use transgenic mouse models, "omic" technologies such as single cell sequencing and mass spectrometry, as well as the culture of primary and cancer cells. In addition, we are developing molecules (antisense oligonucleotides) to reduce tumor growth in pre-clinical models.

Publications

2023

Rhéaume K#, Chen Z#, Wang Y#, Plante C, Hewa Bostanthirige D, Lévesque M, Geha S, Le LQ and Brosseau JP$ (2023). Whole central and peripheral nervous system mice dissection. Journal of Visualized Experiments. (192), e64974

2021

Mohamad T, Plante C, and Brosseau JP$ (2021). Toward understanding the mechanism of malignant peripheral nerve sheath tumor. International Journal of Molecular Sciences. 22(16) :8620

Brosseau JP$#, Sathe AA#, Wang Y, Nguyen T, Glass DA, Xing C, and Le LQ$. Human Cutaneous Neurofibroma Matrisome Revealed by SingleCell RNA Sequencing. Acta Neuropathologica Communications. 2021 9(1)

Yao H, Li C, He F, Song T, Brosseau JP, Wang H, Lu H, Fang C, Shi H, Lan J Zhang Y, Fang JY and Xu J. (2021). A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chemical Biology. 2(1):192-205

2020

Brosseau JP. (2020). Regulations on messenger RNA: wires and nodes. Advances in Experimental Medicine and Biology. 1248: 251-264

Xu J, Brosseau JP and Shi H. (2020). Targeted degradation of immune checkpoint proteins: emerging strategies for cancer immunotherapy. Oncogene 39(48):7106-7113

Chen Z, Li S, Mo J, Hawley E, Wang Y, He Y, Brosseau JP, Shipman T, Clapp DW, Carroll TJ, Le LQ. Schwannoma Development is Mediated by Hippo Pathway Dysregulation and Modified by RAS/MAPK Signaling. JCI Insight. 2020 5(20)

Brosseau JP$, Liao CP and Le LQ$. Translating current basic research into future therapies for Neurofibromatosis type I. British Journal of Cancer. 2020 123: 178-87

2019

Brosseau JP$ and Le LQ$. Heterozygous Tumor Suppressor Microenvironment in Cancer Development. Trends in Cancer 2019(9):541-6

Yao H, Lan J, Li C, Shi H, Brosseau JP, Wang H, Lu H, Fang C, Zhang Y, Liang L, Zhou X, Wang C, Xue Y, Cui Y and Xu J. Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumors. Nature Biomedical Engineering 2019;3(4):306-17

Chen Z, Mo J, Brosseau JP, Shipman T, Wang Y, Liao CP, Allaway RJ, Gosline SJC, Guinney J and Le LQ. Spatiotemporal Loss of NF1 in Schwann Cell Lineage Leads to Different Types of Cutaneous Neurofibroma Susceptible to Modification by the Hippo Pathway. Cancer Discovery 2019;9(1):114-29

2018

Brosseau JP. Splicing Isoform-Specific Functional Genomic in cancer cells. Applied Cancer Research 2018;38:18

Brosseau JP, Liao CP, Wang Y, Ramani V, Vandergriff T, Lee M*, Patel A*, Ariizumi K and Le LQ. NF1 Heterozygosity Fosters de Novo Tumorigenesis, but Impairs Malignant Transformation. Nature Communications 2018;9(1):5014

Liao CP, Booker R, Brosseau JP, Chen Z, Mo J, Tchegnon E, Wang Y, Clapp W, and Le LQ. Contribution of Inflammation and Tumor Microenvironment to Neurofibroma Tumorigenesis. Journal of Clinical Investigation 2018;128(7):2848-61

Brosseau JP#, Pichard DC#, Legius E, Wolkenstein P, Lavker RM, Blakeley JO, Riccardi VM, Verma S, Brownell I, and Le LQ. The Biology of Cutaneous Neurofibroma. Neurology 2018;91(Suppl 1):S14-20

2014

Brosseau JP, Lucier JF, Lamarche AA, Shkreta L, Gendron D, Lapointe E, Thibault P, Paquet E, Perreault JP, Elela SA, and Chabot B. Redirecting Splicing With Interfering and Stimulating Bifunctional Oligonucleotides. Nucleic Acids Research 2014;42(6):e40

Brosseau JP, Lucier JF, Nwilati H, Thibault P, Garneau D, Gendron D, Durand M, Couture S, Lapointe E, Prinos P, Klinck R, Chabot B, Perreault JP, and Elela SA. Tumor Microenvironment Associated Modifications of Alternative Splicing RNA 2014;20(2):189-201

2013

Lecka J, Gillerman I, Fausther M, Salem M, Munkonda MN, Brosseau JP, Cadot C, Martín-Satué M, d'Orléans-Juste P, Rousseau E, Poirier D, Künzli B, Fischer B, Sévigny J. 8-BuS-ATP Derivatives as Specific NTPDase1 Inhibitors. British Journal of Pharmacology 2013;169(1):179-96

Venables JP, Brosseau JP, Gadea G, Klinck R, Prinos P, Beaulieu JF, Lapointe E, Durand M, Thibault P, Tremblay K, Rousset F, Tazi J, Elela SA, and Chabot B. RBFOX2 Enforces the Mesenchymal Splicing Signature in Normal and Cancer Tissues. Molecular and Cellular Biology 2013;33(2):396-405

Meet the Team

Jean-Philippe Brosseau

Principal Investigator

As an Assistant Professor in the Department of Biochemistry and Functional Genomics, he is currently driving a research program to elucidate the functional role of the extracellular matrix in tumor progression, mainly using animal models recapitulating Neurofibromatosis Type I tumors. His lab's overarching goal is to understand tumoral progression better and develop a novel line of anti-cancer therapy.

jean-philippe.brosseau@usherbrooke.ca

Dhanushka Jeewantha Hewa Bostanthrige

Post-Doctoral Fellow

Dhanushka initially completed an MD degree at the University of Peradeniya, Sri Lanka, and act as a general surgeon until 2014. He then earned a Ph.D. in Cell and Molecular Biology from Clark Atlanta University, USA for his thesis entitled: ``Inactivation of ID4 promotes prostate cancer stemness``. His PhD was followed by a one-year postdoc at St-Jude Research Children's Hospital in 2020. Dhanushka join our lab in May 2023 as a CRCHUS postdoc fellow

dhanushka.hewa.bostanthirige@usherbrooke.ca

Camille Plante

Graduate Student

While enrolled in the BSc in Biochemistry at UdeS, Camille performed 2 internships in our lab during winter 2021 and summer 2022. Since January 2023, she started her master on a project toward better understanding the genotype-phenotype relationship in NF1.

camille.plante@usherbrooke.ca

Opeoluwa ALLI-OKE

Graduate Student

Opeoluwa had her undergraduate and master’s degrees at the University of Lagos, Nigeria. She’s currently undergoing another master’s degree program at the Department of Biochemistry and Functional Genomics, UdeS under an Abdenour-Nabid, MD scholarship. Her research interest is in Oncology and she’s currently working on understanding the role of an extracellular factor (TGF-β1) in driving the intracellular mechanism (alternative splicing) of fibroblast differentiation to myofibroblast.

opeoluwa.alli-oke@usherbrooke.ca

Social Activities

May 2023

Contact Us

Address

Contact

Opening Hours

We are hiring at all levels! Please send your cv and cover letter

3001 12 Ave N, Sherbrooke
Quebec J1H 5H3

jean-philippe.brosseau@usherbrooke.ca

Mon - Fri

8:00 am – 5:00 pm (EST)